Part:BBa_K1875012:Design

This part produces a guide RNA that pairs with an operator.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 247
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part produces the guide in BBa_K1875006 and the guide that matches the target sequence on BBa_K1875015

Source

This plasmid was based off of the work by Feng Zhang at MIT and the x330 plasmid